The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live‐cell imaging. In particular, the development of FPs for fluorescence (or Förster) resonance energy transfer (FRET) microscopy is providing important tools for monitoring dynamic protein interactions inside living cells. The increased interest in FRET microscopy has driven the development of many different methods to measure FRET. However, the interpretation of FRET measurements is complicated by several factors including the high fluorescence background, (...) the potential for photoconversion artifacts and the relatively low dynamic range afforded by this technique. Here, we describe the advantages and disadvantages of four methods commonly used in FRET microscopy. We then discuss the selection of FPs for the different FRET methods, identifying the most useful FP candidates for FRET microscopy. The recent success in expanding the FP color palette offers the opportunity to explore new FRET pairs. (shrink)

Alfred D. Chandler, Jr. is a worthy successor to Joseph Schumpeter as analyst of the large corporation and its role in economic growth. His new book, Scale and Scope, a comparative history of corporate capitalism in the U. S., Britain, and Germany, is animated by a vision of the large corporation as the leading force in economic growth, outdistancing older owner?managed forms of organization with a superior ability to invest entrepreneurially in large?scale production, mass distribution, and professional management. Chandler's account (...) implicitly relies on a dynamic?capabilities? theory of competition that reveals the fundamental irrelevance of neoclassical theory and policy, including antitrust policy. In some ways, however, Chandler's vision is too narrow, underplaying the role of markets in economic growth. One needs to know how economic systems build capabilities, not merely how the corporate institution does so. Moreover, there is reason to think that the last few decades have seen an organizational revolution with a dynamic rather different from the one that animates Chandler's account. As a result, Chandler's work is important for understanding the present?day issues of industrial competitiveness, but is only one piece of the puzzle. (shrink)

n the pioneering days of radio, my grandfather's job was to lecture to young engineers who were joining Marconi's company. To illustrate that any complex wave form can be broken down into summed simple waves of different frequencies (important in both radio and acoustics), he took wheels of different diameters and attached them with pistons to a clothesline. When the wheels went round, the clothesline was jerked up and down, causing waves of movement to snake along it. The wriggling clothesline (...) was a model of a radio wave, giving the students a more vivid picture of wave summation than mathematical equations could ever have done. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:The Object of French Studies GebrauchkunstRichard Klein (bio)If I may say something about the title—it points to the possibility that we are discussing the nature and future of French studies at the precise moment that France is about to disappear. There are those who believe that on January 1, 1999, when the euro becomes the common currency of the European Union, France will become a province of Germany. Effectively (...) real sovereignty will pass from Paris to Frankfurt.Already there are those at Cornell, for example, who think that we, in the French section, should seek to merge with the German department. I support the move (which isn’t going to happen), if only as a permanent reminder to avoid the fetishism of French. In today’s world, French is a question more than an answer to any question. French studies should be open even to the possibility that, these days, to speak French, it is becoming more and more necessary to speak German. The opposite has been true for a long time, as the French smugly know all too well—having for centuries lent their language of refinement and savoir-vivre to the Huns. The imminence of the conjuncture should remind us, too, that French studies is itself situated within the history of French—is transformed in its possibilities and embodiments by that history and by what is happening now, as we say, historically. Finally, the point here is to recall that the question of French studies has a history, that it is not merely being invented at this moment, but also reactivated as an object of discussion and debate.In fact, the current debate has been going on for some time. In 1993–94 the issue was addressed at two national conferences: a two-part symposium was organized by the Committee for the Future of French Studies at the University of Pennsylvania and the University of Wisconsin; another was held at New York University organized by the Association for French Cultural Studies and the Institute of French Studies. Summer institutes at Dartmouth and Northwestern have dealt with the issue. There is a large and growing bibliography. I cite some of the most prominent recent articles—to judge by how frequently they are cited in others I have read:—Emily Apter, “Comparative Exile: Competing Margins in the History of Comparative Literature.”—Lawrence Kritzman, “Identity Crises: France, Culture, and the Idea of the Nation.—Marie-Pierre Le Hir, “French Cultural Studies in the United States.”—Sandy Petrey, “French Studies/Cultural Studies: Reciprocal Invigoration or Mutual Destruction?”—Kristen Ross, Fast Cars, Clean Bodies: Decolonization and the Reordering of French Culture (often cited as the best example we have of what French studies might be).—Kristen Ross, “World Literature and Cultural Studies.”—Thomas Pavel, “Les études culturelles: Une nouvelle discipline?” Many questions have been raised in these articles about the possibility and necessity of doing French studies. There are those here who take it for granted that one can identify something French, can locate whatever it is that may be said to participate in a specifically French identity. Some, like Pavel, see French studies (and a fortiori so-called cultural [End Page 5] studies) as an excuse for doing n’importe quoi; it defies definition and merely authorizes more confusion and incoherence. Others, like Kritzman, have a more optimistic notion of how one could define French studies without abandoning some notion of the specificity and uniqueness, the irreducible identity of what is called French.Some critics, like Ross and Le Hir, tend to see French studies as inevitably serving to dissolve any particularism associated with French. What is called French culture has been irretrievably pluralized by the effects of decolonization, by the multiplication of francophonies and by their insertion into an Americanized system of globalization. Kids in suburbs of Paris and Cairo wear American baseball hats turned around and grow up eating McDonald’s. Where is the French in that? The object of French studies may be nothing but France’s assimilationist tendency to colonize others, to claim a universal mission, even as it is transformed by its own failed colonial history and by the very diversity it aims to... (shrink)

Robert Grosseteste was a prolific theological and scientific writer, a translator, bishop of Lincoln , and a candidate for sainthood. There have been several studies of his life and his works, the most recent being that of Richard Southern. Nevertheless, James McEvoy's comment in 1983 that “the course of Grosseteste's life up until 1225 is almost completely unknown” remains largely true. The problem is common in medieval history—there is a dearth of reliable sources for the subject's early life. I (...) do not intend to discuss here Grosseteste's youth, or even his life until 1198 . I will accept, with a few reservations, the model Southern presented for Grosseteste's life until 1198 — that Grosseteste came from an undistinguished family, acquired an education, and may have taught in local schools; after serving the bishop of Lincoln in a minor capacity, he managed to obtain an administrative position under Bishop de Vere in the diocese of Hereford. Unfortunately, Bishop de Vere died on Christmas Day 1198, and his household was disbanded. It is the period following 1198 until 1225 that needs scrutiny. The recent discovery of a reference to Robert Grosseteste in a thirteenth-century cartulary demands that we reconsider the way in which historians have structured Grosseteste's career, both because of what this manuscript records and because of the light it sheds upon the methodology that has underpinned Grosseteste scholarship. (shrink)

This book presents selections from the political and social thought of the ancient West from the early sixth century BCE up to the early years of the Roman Empire and includes not only the classic philosophers, Plato, Aristotle, and Cicero, but a number of dramatists and historians as well. The range of topics these writings treat run from class conflict, through the perils of democracy and the horrors of tyranny, to the place of women in politics, while the styles range (...) from the deeply dramatic of Sophocles' Antigone and the bawdy satire of Aristophanes' Assemblywomen to Plato's Socratic dialogue Republic and Aristotle's scientific treatise Politics. The translations have been chosen, and sometimes modified, for clarity and readability, and are accompanied by introductions which set forth the historical context and trace the general lines of thought the readings develop. Frequent notes explain references to ancient lore unfamiliar to many readers. Questions for discussion accompany each reading. (shrink)

PLATO'S UNIVERSE by Gregory Vlastos. Clarendon Press: O.U.P., 1975. xiii+130 pp. £3.75.

This essay contends that Jonathan Bennett gave a passive reading of conatus, and that he misunderstood Spinoza’s conception of mental representation, mistakenly attributing to Spinoza the common, contemporary view that representational content does not supervene on the intrinsic features of representations. A reading of the conatus as an active, motive principle of opposition is presented. It is argued that Spinoza’s notion of representation is best understood as grounded in a conception of causation on which effects bear intrinsic, distinctive structural marks (...) of their causes. (shrink)

This book deals with the formation of Christian conscience in modern Africa....It can be divided into three parts. The first part is a sociological review on urbanization in Africa. The second part presents a theological reflection on conscience. The third part presents a general survey of the pastoral problems facing the Church in Africa, and in particular the urban centers. The dichotomy between the formation of conscience and the actual life experienced by a considerable number of Christians in Africa today (...) is a crucial moral and pastoral challenge. The author makes the recommendation that African realities should determine the priorities in inculturation and should provide the context for a move towards African Christian conscience. (back cover). (shrink)

Reviews in allegory the approaches taken to the problem of agency by contemporary perspectives in psychology and broader intellectual tradition. The author argues that agency can only be rendered sensible or possible where there is freedom from traditional determinisms and where there is real moral content. It is argued that agency will only be possible when moral relativity is overcome. 2012 APA, all rights reserved).

It is widely assumed that selection history accounts of function can support a fully reductive naturalization of functional properties. I argue that this assumption is false. A problem with the alternative causal role account of function in this context is that it invokes the teleological notion of a goal in analysing real function. The selection history account, if it is to have reductive status, must not do the same. But attention to certain cases of selection history in biology, specifically those (...) involving meiotic drive, shows that selection historical explanations are available in the case of items without any plausible function. Making contributions to goals, here fitness, must be introduced as an additional constraint in the analysis, either explicitly, or implicitly by appeal to individual functions or the selection-of/selection-for distinction. (shrink)

Donald Davidson argues that his interpretivist approach to meaning shows that accounting for the intentionality and objectivity of thought does not require an appeal, as John McDowell has urged it does, to a specifically rational relation between mind and world. Moreover, Davidson claims that the idea of such a relation is unintelligible. This paper takes issue with these claims. It shows, first, that interpretivism, contra Davidson's express view, does not depend essentially upon an appeal to a causal relation between events (...) in the world and speakers' beliefs. Second, it shows that interpretivism essentially, if implicitly, depends upon interpreters' appealing to facts taken in in perception, and that such facts are suited to provide a rational connection between mind and world. The paper then argues that none of Davidson's legitimate epistemological arguments tell against the idea that experience, in the form of the propositional contents of perception, can play a role in doxastic economy. Finally, it argues that granting experience such a role is consistent with Davidson's coherentist slogan that nothing can count as a reason for holding a belief except another belief. (shrink)

This paper is a result of remarks delivered at the 2014 conference of the Florida Philosophical Association during a book symposium on Elijah Chudnoff's Intuition.

The topic of the present essay is the proper understanding of Donald Davidson’s version of the publicity requirement for the determinants of linguistic meaning. On the understanding I promote, the requirement is very strict indeed. My narrow aim is to show how such a strict conception of the publicity requirement can be maintained despite the evident need for interpreters to go beyond what is public on that conception in the process of constructing Davidsonian theories of meaning. Towards that aim, I (...) engage dialectically with treatments of Davidson’s principle of charity owing to Lepore and Ludwig and to Bar-On and Risjord, each of which, in different ways, recommend a more permissive approach to the publicity requirement than the one I recommended here. A broader aim is to shed some light on what would be required to take seriously the larger ambitions of Davidson’s semantic program. (shrink)

Through personal testimony, the author details the experience of fathering a baby with a poor prenatal diagnosis. The author invites the reader to follow his journey, from learning his wife is pregnant, through their experiences as a family with their unborn daughter’s poor prenatal diagnosis, welcoming their baby girl at her birth, and ultimately finding peace in her early passing. Perinatal peer support is discussed and encouraged, drawing attention to the needs and concerns of the babies, women, and families who (...) may not know to seek help in a similar situation. Great honor is given to the beauty and sanctity of life from the perspective of a father with a sick unborn child. National Catholic Bioethics Quarterly 14.1 : 31–37. (shrink)